iFluor™ dyes are a series of highly water-soluble fluorescent dyes. Conjugates made with iFluor™ dyes exhibit superior brightness and photostability, outperforming Alexa Fluor® conjugates and other spectrally similar conjugates in certain applications.
Superior Replacement for Tyramide Signal Amplification (TSA)
Human lung adenocarcinoma positive tissue sections were stained with mouse anti-EpCam antibody and then followed by PSA™ method usingiFluor 488™ PSA™ Imaging Kit with Goat Anti-Mouse IgG(Cat#45260) or TSA method using Alexa Fluor® 488 tyramide respectively. Images showed that PSA™ super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor® 488 TSA method. Cell nucleus were stained with Nuclear Blue™ DCS1 (Cat#17548).
Key Features of iFluor™ Styramide™:
Exceptional sensitivity: >100-fold enhancement over standard IHC, ICC, IF
Higher reactivity: a faster, more efficient & robust labeling of styramide conjugates at the HRP-target interaction site
Conserve precious antibodies: allow use of significantly less primary antibodies or probe without any sacrifice in detection sensitivity