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Doron Scientific > Products > Promotions > AAT Bioquest Reactive Oxygen Species (ROS) Detection

AAT Bioquest Reactive Oxygen Species (ROS) Detection

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Description

AAT Bioquest Reactive Oxygen Species (ROS) Detection: Highly Sensitive & Selective ROS Reagents and Assay Kits

AAT Bioquest offers the most complete product line for detecting ROS. Our ROS products include novel fluorescent indicators and assay kits to detect various reactive oxygen species and related biochemical molecules with ultimate sensitivity and high selectivity. They are optimized for multiplexing applications by selecting desired excitation and emission wavelengths for flow cytometric analysis and fluorescence imaging of live cells from a broad spectrum of our ROS detection products.

Figure 1. Detection of ROS in Jurkat cells with Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit. Jurkat cells were seeded on the same day at 300,000 cells/100µL/well in a Costar black wall/clear bottom 96-well plate. The ROS assay loading solution (100 µL/well) was added and incubated in a 5% CO2, 37 °C incubator for 1 hour. And then the cells were treated with 1mM, 0.1mM H2O2 or without H2O2 for 30 minutes. The fluorescence signal was monitored at Ex/Em = 490/525 nm (cutoff at 515 nm) with bottom read mode using FlexStation (Molecular Devices).
Learn More about Our ROS Products

Intracellular Hydrogen Peroxide Probes
  DCFH-DA Dihydrorhodamine 123 OxiVision™ Blue OxiVision™ Green
Principle Esterases cleave off diacetate group to yield DCFH which is oxidized by H2O2 to DCF and fluoresces green upon excitation Oxidation by H2O2 yields rhodamine 123, which fluoresces blue upon excitation OxiVision™ probes are oxidized by intracellular H2O2. Generates fluorescence upon excitation.
Ex/Em (nm) 504/525 507/527 405/450 497/516
Filter Set FITC FITC DAPI FITC
Live Cells Yes
Imaging Yes
HCS Yes
Flow Cytometry Yes
Unit Size 25 mg 10 mg Kit (100 Tests) Kit (100 Tests)
Cat No. 15204 15206 11504 (Imaging)
11505 (Flow)
11503 (Imaging)
11506 (Flow)
Intracellular ROS Selection Guide
ROS Species ROS Brite™ 570 ROS Brite™ 670 ROS Brite™ 700 ROS Brite™ HDCF Amplite™ ROS Green Amplite™ ROS Red
Hydrogen Peroxide (H2O2) + + + +++ +++ +++
Hydroxyl radical (•OH) ++ ++ ++ + + +
Tert-butyl-hyrdoperoxide (TBHP) + + + + + +
Hypochlorous acid (HOCl) – + ++ – + –
Superoxide anion (O2•-) + ++ ++ – – –
Nitric Oxide (NO) – – – – – –
Peroxynitrite anion (ONOO–) – – – – – –
Cat No. 22902 22903 16004 16053
22900
22904
22901
ROS-Selective Probes and Assay Kits
ROS Species OxiVision™ Green OxiVision™ Blue MitoROS™ 520 MitoROS™ 580 MitoROS™ OH580
Hydrogen Peroxide (H2O2) +++ +++ – – –
Hydroxyl radical (•OH) – – – – +++
Tert-butyl-hyrdoperoxide (TBHP) – – – – –
Hypochlorous acid (HOCl) – – – – –
Superoxide anion (O2•-) – – +++ +++ –
Nitric Oxide (NO) – – – – –
Peroxynitrite anion (ONOO–) – – – – –
Cat No.
11503
11506
11504
11505
16060
22970
22971
16055

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