In clinical molecular diagnostics, nucleic acid amplification tests such as the reverse transcriptase-polymerase chain reaction (RT-PCR) enable the early detection and identification of infectious diseases caused by retroviruses. Utilizing mRNA as its starting template, RT-PCR expeditiously transcribes, amplifies and detects the genetic material of retroviruses in vitro. Nucleic acid tests have been successfully used in the detection of well-known retroviruses including influenza viruses, enteroviruses, coronavirus, Ebola virus, and HIV.
Quantification of RT-PCR products can be generally divided into two categories: end-point RT-PCR and real-time PCR. The use of end-point RT-PCR is preferred for measuring gene expression changes in small number of samples, while real-time RT-PCR has become the standard method for validating gene expression changes on a global scale. Real-time PCR (RT-PCR) fluorescent reporter molecules include dyes that bind to the double-stranded DNA, such as Cyber Green™, or sequence-specific paired FRET probes, such as Tide Fluor™ and Tide Quencher™ dyes.
(Note that AAT Bioquest provides reagents intended for research use only)
AAT Bioquest’s products are used by researchers in universities and diagnostic and drug discovery companies, as tools for the development of COVID-19 assays, screening and vaccine research, including our newly-developed Covidyte™ series. We have partnered with some of the largest companies in the biotechnology and life sciences industry in an effort to support the global community during this health crisis.