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Biotinylated Human FGF basic Protein, Avitag™,His Tag (MALS verified)

Product specifications

Biotinylated Human FGF basic, Avitag,His Tag (FGC-H81E3) is expressed from E. coli cells. It contains AA Pro 143 – Ser 288 (Accession # P09038-4).

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE).

Catalog Number

FGC-H81E3-200ug

Product Category

Proteins & Peptides

Material Category

Cytokines&Growth Factors

Supplier

ACROBiosystems

Storage Tempreture

-20℃

Species

Human

Host

E. coli

Product Type

Cytokines&Growth Factors

Format

Powder

Purity

90%

Conjugate

Biotin-labeled

Bioactivity

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.

Molecule

FGF basic

Tag

N-Avi & N-10×His

Expression System

E. coli

Expregion

Pro 143 - Ser 288

Endotoxin Level

1.0 EU per μg

Buffer

50 mM Tris,150 mM NaCl,pH7.5

Product Size & Price

Product size: 200ug

Product price: 1080

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Order Details

We are continuously updating our catalog with advanced verified products. please send your request of our latest COA&SDS datasheet

Limitations

Please note: All products are “FOR RESEARCH USE ONLY”. NOT FOR USE IN DIAGNOSTIC PROCEDURES or Human or Veterinary or Food.

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