Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer’s disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 20.2 kDa. The protein migrates as 20-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteins & Peptides
MABSol® Biotin Labeled Proteins
Biotinylated Mouse TNF-alpha Protein, His,Avitag (TNA-M82E9) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804).
C-8×His & C-Avi
Leu 80 - Leu 235
1.0 EU per μg