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Biotinylated Recombinant Protein L, His,Avitag™ (MALS & FACS verified)

Product specifications

Biotinylated Recombinant Protein L, His,Avitag, also known as Recombinant Peptostreptococcus magnus Protein L, was expressed in E. coli at ACRObiosystems.

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 43.7 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE). This protein can bind to VL-Kappa.

Catalog Number

RPL-P81Q7-100ug

Product Category

Proteins & Peptides

Material Category

MABSol® Biotin Labeled Proteins

Supplier

ACROBiosystems

Storage Tempreture

-20℃

Species

Peptostreptococcus magnus

Host

E. coli

Product Type

MABSol® Biotin Labeled Proteins

Format

Powder

Purity

95%

Conjugate

Biotin-labeled

Bioactivity

Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Molecule

Protein L

Tag

N-6×His & N-Avi

Expression System

E. coli

Expregion

Lys 106- Gly 470

Endotoxin Level

1.0 EU per μg

Buffer

PBS, pH7.4

Product Size & Price

Product size: 100ug

Product price: 319

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Order Details

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Limitations

Please note: All products are “FOR RESEARCH USE ONLY”. NOT FOR USE IN DIAGNOSTIC PROCEDURES or Human or Veterinary or Food.

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