Product specifications

HCoV-229E Spike Protein, His Tag (SPN-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 16 – Pro 1115 (Accession # P15423-1 (TI 871-872 PP)).The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus. Proline substitutions TI 871-872 PP are introduced to stabilize the trimeric prefusion state of the spike protein.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 125.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Catalog Number

SPN-H52H3-500ug

Product Category

Proteins & Peptides

Material Category

Viral Proteins

Supplier

ACROBiosystems

Storage Tempreture

-20℃

Species

HCoV-229E

Host

HEK293

Product Type

Viral Proteins

Format

Powder

Purity

90%

Conjugate

Unconjugated

Bioactivity

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecule

Spike protein

Tag

C-10×His

Expression System

HEK293

Expregion

Cys 16 - Pro 1115

Endotoxin Level

1.0 EU per μg

Buffer

PBS

Product Size & Price

Product size: 500ug

Product price: 3080

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Limitations

Please note: All products are “FOR RESEARCH USE ONLY”. NOT FOR USE IN DIAGNOSTIC PROCEDURES or Human or Veterinary or Food.

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