Product specifications

Human Akt1, His,Strep II Tag (AK1-H5283) is expressed from human 293 cells (HEK293). It contains AA Met 1 – Ala 480 (Accession # AAH00479).

This protein carries a polyhistidine tag at the C-terminus, followed by a twin strep tag. The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Catalog Number

AK1-H5283-500ug

Product Category

Proteins & Peptides

Material Category

Other Recombinant Proteins

Supplier

ACROBiosystems

Storage Tempreture

-20℃

Species

Human

Host

HEK293

Product Type

Other Recombinant Proteins

Format

Powder

Purity

92%

Conjugate

Unconjugated

Bioactivity

RAC-alpha serine/threonine-protein kinase (AKT1) is also known PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt and RAC-PK-alpha, which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily and is expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase).

Molecule

Akt1

Tag

C-6×His & C-Twin-Strep

Expression System

HEK293

Expregion

Met 1 - Ala 480

Endotoxin Level

1.0 EU per μg

Buffer

20 mM Tris, 150 mM NaCl, pH8.0

Product Size & Price

Product size: 500ug

Product price: 1600

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Limitations

Please note: All products are “FOR RESEARCH USE ONLY”. NOT FOR USE IN DIAGNOSTIC PROCEDURES or Human or Veterinary or Food.

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