, , , , ,

Human CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)

Product specifications

Human CD73 Protein, His Tag (CD3-H52H7) is expressed from human 293 cells (HEK293). It contains AA Trp 27 – Ser 549 (Accession # P21589-1).

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 40 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Catalog Number

CD3-H52H7-500ug

Product Category

Proteins & Peptides

Material Category

Enzymes

Supplier

ACROBiosystems

Storage Tempreture

-20℃

Species

Human

Host

HEK293

Product Type

Enzymes

Format

Powder

Purity

90%

Conjugate

Unconjugated

Bioactivity

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Molecule

CD73

Tag

C-10×His

Expression System

HEK293

Expregion

Trp 27 - Ser 549

Endotoxin Level

1.0 EU per μg

Buffer

20 mM Tris, 120 mM NaCl, pH7.5

Product Size & Price

Product size: 500ug

Product price: 1320

Add to quote

Shipping condition & fees

All prices are exclusive of duties, taxes, customs & Shipping fees

Shipping & Handling

Our customer service will inform you about the expected delivery time.

Order Details

We are continuously updating our catalog with advanced verified products. please send your request of our latest COA&SDS datasheet

Limitations

Please note: All products are “FOR RESEARCH USE ONLY”. NOT FOR USE IN DIAGNOSTIC PROCEDURES or Human or Veterinary or Food.

Get a quote