, , , , ,

SARS-CoV-2 Spike Trimer Protein (T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N), His Tag (MALS verified)

Product specifications

SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Ha) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 – Pro 1213 (Accession # QHD43416.1). The mutations T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N were identified in the SARS-CoV-2 Mu variant (Pango lineage: B.1.621). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138 kDa. The protein migrates as 150-180 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Catalog Number

SPN-C52Ha-500ug

Product Category

Proteins & Peptides

Material Category

Viral Proteins

Supplier

ACROBiosystems

Storage Tempreture

-20℃

Species

SARS-CoV-2

Host

HEK293

Product Type

Viral Proteins

Format

Powder

Purity

95%

Conjugate

Unconjugated

Bioactivity

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecule

Spike protein

Tag

C-10×His

Expression System

HEK293

Expregion

Val 16 - Pro 1213

Endotoxin Level

1.0 EU per μg

Buffer

PBS

Product Size & Price

Product size: 500ug

Product price: 3080

Add to quote

Shipping condition & fees

All prices are exclusive of duties, taxes, customs & Shipping fees

Shipping & Handling

Our customer service will inform you about the expected delivery time.

Order Details

We are continuously updating our catalog with advanced verified products. please send your request of our latest COA&SDS datasheet

Limitations

Please note: All products are “FOR RESEARCH USE ONLY”. NOT FOR USE IN DIAGNOSTIC PROCEDURES or Human or Veterinary or Food.

Get a quote