Human Siglec-3 / CD33 Protein, His Tag (MALS verified)
Product specifications
Human Siglec-3, His Tag (CD3-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 18 – His 259 (Accession # AAH28152.1).
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.6 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Catalog Number
CD3-H5226-100ug
Product Category
Proteins & Peptides
Material Category
Bio-Markers & CD Antigens
Supplier
ACROBiosystems
Storage Tempreture
-20℃
Species
Human
Host
HEK293
Product Type
Bio-Markers & CD Antigens
Format
Powder
Purity
95%
Conjugate
Unconjugated
Bioactivity
Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Iglike lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
Molecule
Siglec-3
Tag
C-6×His
Expression System
HEK293
Expregion
Asp 18 - His 259
Endotoxin Level
0.1 EU per μg
Buffer
PBS, pH7.4
