Mouse CD73 / NT5E Protein, His Tag (active enzyme)
Product specifications
Mouse CD73, His Tag (CD3-M52H9) is expressed from human 293 cells (HEK293). It contains AA Trp 29 – Ser 551 (Accession # Q61503-1).
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Catalog Number
CD3-M52H9-20ug
Product Category
Proteins & Peptides
Material Category
Enzymes
Supplier
ACROBiosystems
Storage Tempreture
-20℃
Species
Mouse
Host
HEK293
Product Type
Enzymes
Format
Powder
Purity
90%
Conjugate
Unconjugated
Bioactivity
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Molecule
CD73
Tag
C-10×His
Expression System
HEK293
Expregion
Trp 29 - Ser 551
Endotoxin Level
1.0 EU per μg
Buffer
20 mM Tris, 120 mM NaCl, pH7.5
